Used INVITROGEN Neon MPK 5000

Thermo Fisher Invitrogen MPK5000 Neon Transfection System

The Neon Transfection System enables fast and efficient delivery of nucleic acids into all mammalian cell types, including primary, stem, and difficult-to-transfect cells. The flexible and open system allows the user to perform high-quality transfections using optimized or user-defined protocols in three simple steps with as few as 2 104cells per reaction. A novel reaction chamber provides a dramatic increase in transfection efficiency and cell viability. The Neon Transfection System is:

Efficientup to 90% in many cell types, including difficult-to-transfect cells, primary, and stem cells

Flexibleeasily transfect from 2 104cells to 6 106cells per reaction

Simpleeasy to use, with a single reagent kit for all cell types

Versatileopen system allows electroporation parameters to be optimized freely

One-of-a-kind electroporation

Unlike standard cuvette-based electroporation chambers, the Neon system uses a patented, biologically compatible pipette tip chamber that generates a more uniform electric field. Neon Tips are components of the Neon Transfection System Kits, available in 100 L and 10 L formats for large- and small-scale transfections. This design allows better maintenance of physiological conditions, resulting in very high cell survival compared to conventional electroporation (see figure).

Starting Material Amount:2Г—10’4 – 6 x10’8 cells; Varies by cell type.