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Self-contained units for temperature cycling in test samples, using water, metal blocks, and/or conductive fluids. These incubators are used for heating/cooling cycles in cell culture studies, DNA sequencing, and gene manipulation.
1. The thermocycling laboratory incubator instrument's throughput and reaction success are affected by some thermal cycled features, which ultimately affect time and cost savings.
2. When determining the laboratory hybridization incubator's throughput, the reaction time is important.
3. The overall cycling time depends largely on the temperature uniformity of the sample block or chamber. If a sample requires one minute at 95
4. Before the samples reach the same temperature as the block or chamber, there will be some lag time, and therefore, temperature uniformity should be measured by sample temperature, not block or chamber temperature.
5. Another important feature of the thermocycling laboratory incubator may be dwell time. If reactions are performed overnight, the length of time a device can store samples at a specific temperature becomes important.
6. Facilities should carefully examine various cycling methods before purchasing a laboratory hybridization incubator, with respect to their effect on reaction time and outcome.
7. The capillary air thermal cycles were introduced for the purpose of reducing the total cycling time of a reaction, by increasing the ramp rate uniformity and decreasing the hold times necessary at each step of the polymerase chain reaction cycle.
8. A reaction is performed by thermocycling laboratory incubators in 10 to 30 minutes, while it requires three to five hours to complete a reaction for conventional heat-block cycles. One study, however, indicates that it is possible to achieve similar reaction times in a heat-block cycled by significantly reducing the hold times at each of the three cycle steps and still obtain acceptable results.
9. To prevent evaporation, samples may require oil overlay, which can add extra time to the sample-preparation process and is often messy.
10. Heated lids are now offered by most thermocycling laboratory incubators. These prevent evaporation and eliminate the need to add oil to the samples.
11. Facilities may consider the adaptability of thermal cycled-to-robotics systems with the increasing degree of laboratory automation. Some laboratory hybridization incubators can be adapted fairly easily for robotics applications, which can save time and, in some cases, reduce the number of false-positive results caused by human contamination.